Sami Labs Ltd., Sabinsa’s associated group, has established a cosmeceuticals claims substantiation laboratory at it’s Bangalore research facility.
A summary of the current capabilities of the cell culture and biochemical validation laboratory is as follows:
Facilities available:
- Class 100 safety Hood (Class II level)
- Class 10,000 lab (sterile lab)
- Carbon-dioxide Incubator (thermo)
- Inverted microscope (Olympus) with CCTV and fluorescence attachment
- Microplate reader (BMG, Fluostar Optima) with Absorbance, Luminescence, Fluorescence detectors
- Temperature control Ultra centrifuge(20,000 rpm)
- Cryofreezer (-80oC)
Cell cultures Testing:
1. Toxicity evaluation
- Human fibroblast
- Mutagenicity against bacterial tester strain TA 102
2. Efficacy studies:
- Melanogenesis promoters / inhibitors: Efficacy screening for skin pigmentation promotion or skin lightening, required in validating efficacious agents in vitiligo or skin fairness respectively.
- Melanocyte cell line (Melana A)
- Tyrosinase inhibition assay
- Hyperproliferative studies / psoriasis evaluation
- SVK14 Keratinocytes proliferation, measurement of ATP/lactate dehydrogenase
- SVK14 Keratinocytes proliferation, measurement of ATP/lactate dehydrogenase
- Skin conditioning / anti-wrinkle properties
- Fibroblast proliferation and release of various enzymes and cytokines
- Anti-elastase activity
- Anti-collagenase activity
- Anti-hyaluronidase activity
- Anti-inflammatory activity
- Evaluation of COX inhibition
- Evaluation of COX inhibition
- Wound healing activity
- Proliferation of fibroblasts
- Proliferation of Keratinocytes
- Antioxidant assay
- DPPH radical scavenging activity
- In vitro lipid peroxidation
- Anti-acne/Anti-bacterial activities, Effectiveness against
- Propionibacterium acnes
- Staph. aureus
- Staph. epidermidis
- Strept. mutans
- E.coli
- Bacillus subtilis
- Anti-dandruff activities
- Effectiveness against Pityrosporum ovale
One of the driving factors is the need for development of non-animal based efficacy studies. Alternative testing methods will be developed and validated at this laboratory
